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Detection of microplastics in honey of stingless bee (Heterotrigona itama) and honey bee (Apis mellifera) from Malaysia

A preliminary Malaysian honey survey compared microscopically classified particles between stingless-bee and honey-bee samples and confirmed polyethylene in selected particles.

Updated 2026-10-22

A preliminary Malaysian honey survey compared microscopically classified particles between stingless-bee and honey-bee samples and confirmed polyethylene in selected particles.

Source details

Yusof Shuaib Ibrahim, Muhammad Naim Rosazan, Muhammad Izzelen Izzauddin Mamat, Sabiqah Tuan Anuar, Wahizatul Afzan Azmi. Biodiversitas. 2025.

DOI: 10.13057/biodiv/d260326

Findings

A preliminary Malaysian honey survey compared microscopically classified particles between stingless-bee and honey-bee samples and confirmed polyethylene in selected particles.

Scroll horizontally for methods and limitations.

EndpointSource-reported resultLocator
Heterotrigona itama honey8.18 ± 2.57 microscopy-classified MPs/gPDF p. 4 Table 1; p. 3 results
Apis mellifera honey5.52 ± 1.13 microscopy-classified MPs/gPDF p. 4 Table 1; p. 3 results
Honey particle subsamplesix selected particlesPDF p. 4 FTIR results
Heterotrigona itama honey1.100 ± 0.653 mmPDF p. 4 results

Methods and scope

Twelve honeys, six from each bee species, were digested in triplicate from 10 g portions with hydrogen peroxide after dilution and filtered onto 1.2 µm glass filters. Stereomicroscopy classified and counted objects. Hot-needle testing, SEM, and FTIR were applied to selected objects. Only six objects could be identified by FTIR, all PE. Equipment/solutions were rinsed/filtered, samples covered, airborne blank filter used, and closed room with air filtration reported. Numerical blank counts/subtraction and polymer-specific recovery are not reported in inspected methods/results.

What this source can support

Supports paper-reported microscopy-based counts and PE presence in the selected objects. Whole-count polymer identity and an absence of natural fibers are not established.

Preliminary selection of farm/store honey. Chemical confirmation of only six selected particles; visually classified fibers dominate and cannot all be labeled chemically confirmed PE. Abstract size range differs from results bins and Table 1 means; use results/table-specific size context only. Blank filter mentioned without numerical blank results or correction. Species comparison does not isolate environment, processing, or packaging as cause.

Honey

Reported values and source locations

Reported valueMeasurement basisConditions and size contextOriginal document location
8.18 ± 2.57 microscopy-classified MPs/gMean ± SDMalaysian farm/store honey; triplicate digests of each honey sample
Results categories <0.3, 0.3-0.5, 0.5-1.0, 1.0-5.0 mm; 1.2 µm filter pore is not detection floor
PDF p. 4 Table 1; p. 3 results
5.52 ± 1.13 microscopy-classified MPs/gMean ± SDMalaysian farm/store honey; triplicate digests of each honey sample
Results categories <0.3, 0.3-0.5, 0.5-1.0, 1.0-5.0 mm; 1.2 µm filter pore is not detection floor
PDF p. 4 Table 1; p. 3 results
six selected particlesCount of chemically identified particlesRandom selection constrained by size for FTIR after microscopy and hot-needle screening
Selection constrained by particle size; no validation for all visually counted objects
PDF p. 4 FTIR results
1.100 ± 0.653 mmMean ± reported spread (text does not label SD at this sentence)Microscopic particle dimensions in stingless-bee honey
Results classified in bins extending below 0.3 mm and up to 5 mm; abstract range differs
PDF p. 4 results
Source provenance

Primary PDF SHA-256: e90dfcc638313ae1de894b1fd8b46438a57e7c347590fef1575f6e533d3b3b96

The source record identifies the scope and limits of the primary-document review. Follow the DOI or official source link for the original document; this site does not redistribute the PDF.

Cite this page

Microplastics Reference Library. Detection of microplastics in honey of stingless bee (Heterotrigona itama) and honey bee (Apis mellifera) from Malaysia. Updated 2026-10-22. https://microplasticsindex.com/sources/s-e90dfcc63831

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